LET THIS ROBOT MAKE YOUR ELISAs EASIER
Robots you have known so far basically require to:
Program the instrument and adapt the ELISA protocol, verifying the absence of drift and carry-over. This task could be asked to the robot producer, to the kit producer or being perfomed in house: anyway, it could be a time-consuming, expensive and not fully successfully operation.
Verify the availability of a proper integrated software for session management, data handling and report storing. Sometimes it could be necessary to accept arrangements when the robot is not able to perform a certain curve algorithm.
Dedicate a computer to the instrument.
Perform the sample extraction manually, than select one or two assays per session, open the instrument, prepare all the reagents bottles in a proper order, launch the robot inizialization and then launch the session. It is recommended for the analyst not to go away in case of process errors.
Run the calibration curve of each assay every session.
Collect the results at the end of the session and -sometimes- do further elaborations.
Empty the instrument, cap the bottles, clean the robot.
CHORUS FOOD IS A NEW CONCEPT OF ELISA ROBOT.
Chorus Food is a novel platform of integrated robot, software and kits in a unique format. While common ELISA kits contain the plate and the liquid reagents, Chorus kits are based on all-inclusive cartridges, including one reaction well, one loading slot for the sample and pre-sealed container with sufficient reagents.
Once the sample extract is pipetted in its loading slot, the cartridge is inserted in the machine carousel and automatically recognized thanks to its barcode.
The robot manages up to 30 determinations with no extra manual work, in a complete walk-away mode, providing at the end of the session the printed report.
Quantitative results are obtained for each sample by calculating the ratio between the sample absorbance and memorized standard zero absorbance and interpolating this relative signal on a virtual batch-related master-curve. This means that no cartridges are supposed to be spent for calibrators apart for the zero standard, whose intensity can be stored for many sessions.
1. Insert the cartridges into the carousel.
2. Read the standard 0 barcode, if necessary, and acquire all the information about the master curve.
3. Start the session and walk away.
4. Quantitative results are automatically printed at the end of the session.
Chorus Tox is the first line of cartridges kits for the quantitative analysis of aflatoxin B1, fumonisins, zearalenone and deoxynivalenol in cereals and feed, with an assay time of 30 – 60 minutes and the following Limits of Detections: 1 ppb aflatoxin B1, 750 ppb fumonisins, 10 ppb zearalenone and 200 ppb deoxynivalenol.
Consistent results were obtained when the whole carousel was loaded with mixed assays, no drift was observed indeed. Repeated measurements of the same extract in one session always led a dosage CV < 10%. Good accuracy was obtained by analyzing Reference Materials.
This product line will be soon improved with a kit for total aflatoxins, one for ochratoxin A and one for T2 /HT2 toxin and will become hence a concrete multiplex automatic platform.