Histamine ELISA kit

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Description

ELISA test kit for the quantitative detection of histamine.

Formats

96 determinations ...... code CI005

Assay principle

Microplate competitive enzyme immunoassay.

Analysable samples

Fresh fish, Fish meal, Milk, Cheese, Sausage, Wine and Champagne.

Detection limit

Fish meal: 100 ppm
Fresh fish, Sausage, Cheese: 2.5 ppm
Milk: 0.1 ppm
Wine, Champagne: 0.25 ppm
   

Assay time

90 minutes

Sample preparation

Fish meal: Water extraction, cetrifugation, dilution, acylation.

Fresh fish, Sausage, Cheese: Homogenization, water extraction, centrifugation, dilution, acylation.

Milk: Precipitation, acidification, centrifugation, acylation.

Wine, Champagne: Dilution, acylation.

Specificity

Hystamine 100%
3-methyl-histamine < 1%
Tyramine < 1%
L-phenylalanine < 1%
L-histidine < 1%
L-tyrosine < 1%
Tryptamine < 1%
5-hydroxy-indole-acetic acid < 1%
Serotonin < 1%
   

Last update

June 2019