B ZERO AFLA B1

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Description

ELISA test kit for the quantitative detection of aflatoxin B1

Formats

48 determinations...... code MA222

96 determinations...... code MA224

Assay principle

Microplate competitive enzyme immunoassay without standard curve.

Sample quantification through interpolation of inhibition value on virtual curve (master-curve) provided for each batch of kit.

Analysable samples

Cereals (corn, sorghum), High moisture corn, Silage and mash, Feed, Nuts (hazelnuts, pistachios, peanuts, almonds), Dried fruits (raisins, figs), DDGS ("Distillers"), Cottonseed, Soy, Soybean meal.

Detection limit

1 ppb (for all the matrices)

Assay time

15 minutes

Sample preparation

Grinding, methanol - water extraction, filtration.

For Silage and mash: pH adjustment of methanol extracts.

For Cottonseed: grinding and homogenization, methanol - water extraction, sedimentation.

Specificity

Aflatoxin B1 100%
Aflatoxin B2 5%
Aflatoxin G1 19%
Aflatoxin G2 < 1%
   

Advantages

B ZERO is a cost-effective line of ELISA test kits. Thanks to the removal of the calibrators, the analyst is not required to spend wells for the standard curve! Quantitative results are obtained by means of a virtual master-curve that is provided within each kit batch.

Last update

April 2019