5-in-1 ELISA 

How many contaminants could a single food contain?
Ideally, every single commodity and finished product should be verified for a number of molecules that could co-occur. Even focusing only on those substances that could be present with a certain probability in a defined matrix category, a relevant number of analysis should be arranged as well, with a considerable expense of time and money.
Having one single multiplex kit that is able to detect a number of relevant analytes in the same sample extract at a reasonable cost is now an option.

Profilo is the result of Tecna R&D investements of the last five years.

The goal of the project was the development of a flexible, easy to use, reliable platform for the quantitative detection of a little group of contaminants in the same sample. Despite the availability of charming new technologies at the state of the art, we decided to keep it as easy as possible, and developed a multiplex ELISA-spot immunoarray system. The microplates wells were spotted with competitive antigens, thus obtaining an immobilized array matrix in each ELISA well. During the assay, liquid reagents are pipetted like in common ELISAs. At the end of the assay, a color is locally developed onto each “spot”: its intensity is inversely proportional to the contamination of the sample. By interpolation of each relative color signal in a batch master-curve, it is possible to obtain the concentration of each contaminant in the sample.

The assay is extremely easy to be implemented and does not require any specific training. A user-friendly reader is available for digital results. Thanks to the master-curve calibration, the system is very cost-effective, since the whole content of the kit can be used for samples only.

A first application of Profilo is now available for beta-testing and includes an assay for the simultaneous detection of aflatoxin B1, deoxynivalenol, T2 toxin, zearalenone and fumonisins in cereals and feed.